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This study was performed to investigate the effect of recombinant bovine Somatotropin (rbST) treatment on some haemortological in primaparous Kundhi buffaloes. Sixteen primaparous Kundhi buffaloes were divided into two groups keeping 8 animals in each group. Group-I served as control and Group-II was treated with 250 mg of rbST fortnightly for 166 days of 1st lactation. The rbST treatment was started after 60 days of lactation. Blood samples were collected every week during pre-treatment (average 60 days) and post-treatment (average 166 days) of 1st lactation. The findings revealed a non significant variation in the erythrogram whereas, leucogram showed a leucocytosis accompanied by neutrophilia and lymphocytosis. They attributed the increase in total leukocytes and lymphocytes as positive response of immune system of buffaloes to rbST treatment. Haemotological values of Granulocytes (GR#), Lymphocytes (LY#), Mean corpuscular hemoglobin concentration (MCHC), Monocytes (MO#), Mean Platelet Volume (MPV), Platelets (P1t), Pct (%), Platelet distribution width (PDW), and White blood cells (WBC) in rbST treated and non-treated buffaloes were affected during various physiological conditions and weather. Mean values of granulocyte (GR#) was significantly decreased (P<0.01) during parturition and winter weather in rbST treated buffaloes as compare to control group. Mean values of Haemogglobin (Hb) was significantly declined (P<0.01) during parturition, lactation and dry period in rbST treated buffaloes as compare control group. Mean values of lymphocyte was significantly decreased (P<0.01) in rbST treated buffaloes during various physiological condition in rbST treated buffaloes as compare to control group. Mean values of MCV was significantly declined (P<0.05) during conception and parturition in buffaloes. Mean values of PCV was significantly (P<0.01) increased during open days, conception and winter in rbST treated buffaloes as compare to control group. Mean values of platelet distribution were significantly increased (P<0.01) during lactation, dry period, conception, summer and winter in rbST treated group as compare to control group. Red Blood cell values were significantly (P<0.01) increased during lactation and parturition in rbST treated buffaloes as compare to control group. Red blood distribution width (RDW) was significantly (P<0.05) increased after administration of rbST during summer as compare to control group. Whereas, rbST treatment did not significantly affected the values of RDW during other physiological conditions in buffaloes. White blood cells were significantly lower (P<0.01) during various physiological conditions in rbST treated buffaloes as compare to control group. In conclusion, application of rbST produced a non-significant variation in the blood profile in primaparous kundhi buffaloes. But erythrogram and leucogram values showed a leucocytosis accompanied by neutrophilia and lymphocytosis after rbST treatment. Haemotological values of both groups were affected during various physiological conditions and weather.

This research was guided to investigate the effects of supplementation of DL-methionine (DLM) and liquid DL-methionine hydroxy analog free acid (MHA) in diets on productive performance, hematological, blood metabolites and histological parameters in broiler chickens under summer conditions in Sina. A total number of 180 one day old Hubbard broiler chicks, with initial body weight (46.9 ± 2.5 g), were divided into 6 equal treatment groups. The treatment were DL-methionine (DLM) at 0.25% level, acid liquid methionine (ALM) and methionine hydroxyl analogue Ca salt (MHA) at 0.29 and 0.30%, respectively with and without dietary L-Threonine (0.0 and 1.0 g/kg diet). Chicks were raised for 35 days in battery cages under semi – arid conditions (high ambient temperature and relative humidity) in open-side housing. The obtained results indicate: The productive performance of DLM and MHA were not significantly different. Supplementation of DLM and MHA significantly improved final body weight, body weight gain, average daily gain, and feed conversion ratio when compared to the control group (P<0.01). The use of DLM enhanced the plasma methionine concentration (P<0.01) and increased the heterophil/lymphocyte ratio (P<0.05). While MHA elevated the plasma urea and uric acid concentration levels (P<0.05).